High PAR and UV-B radiation-induced differential responses in green and white leaf sectors of Pelargonium zonale in relation to sugar, antioxidative and phenolic metabolism

In this study we investigated the specific effects of high photosynthetically active radiation (PAR) and ecologically relevant UV-B radiation (0.90 W m-2) on antioxidative, phenolic and sugar metabolism in variegated Pelargonium zonale plants. The green-white leaf variegation in these plants presents a suitable model system for examining “source-sink” interactions within the same leaf. High PAR (1350 μmol m-2 s-1) and UV-B radiation induced tissue specific responses in variegated P. zonale leaves. While UV-B radiation had a pronounced effect on phenolic content in the white tissue, high PAR intensity stimulated accumulation of phenylpropanoids and flavonoids with preferential antioxidative vs. UV-screening function in green tissue. High PAR stimulated the increase of antioxidative metabolism in both leaf sections. However, the greater enhancement of ascorbate peroxidase and catalase activities and ascorbate content under HL+UV-B than HL only in green sectors, indicated that UV-B radiation and high PAR synergistically stimulated antioxidative defense. These results indicate that green tissue can be considered as high light acclimated, provided with an efficient defense against potential oxidative pressure under high PAR, along with significant protective role of UV-B radiation. Efficient sugar transport from green to white tissue was stimulated by both UV-B radiation and high PAR intensity. By stimulation of starch and sucrose breakdown and carbon allocation in the form of soluble sugars from “source” (green) tissue to “sink” (white) tissue, UV-B radiation stimulates a compensatory mechanism for phenylpropanoid and flavonoid biosynthesis in white tissue, due to the lack of photosynthesis

B‐GATA factors are required to repress high‐light stress responses in Marchantia polymorpha and Arabidopsis thaliana

GATAs are evolutionarily conserved zinc-finger transcription factors from eukaryotes. In plants, GATAs can be subdivided into four classes, A–D, based on their DNA-binding domain, and into further subclasses based on additional protein motifs. B-GATAs with a so-called leucine-leucine-methionine (LLM)-domain can already be found in algae. In angiosperms, the B-GATA family is expanded and can be subdivided in to LLM- or HAN-domain B-GATAs. Both, the LLM- and the HAN-domain are conserved domains of unknown biochemical function. Interestingly, the B-GATA family in the liverwort Marchantia polymorpha and the moss Physcomitrium patens is restricted to one and four family members, respectively. And, in contrast to vascular plants, the bryophyte B-GATAs contain a HAN- as well as an LLM-domain. Here, we characterise mutants of the single B-GATA from Marchantia polymorpha. We reveal that this mutant has defects in thallus growth and in gemma formation. Transcriptomic studies uncover that the B-GATA mutant displays a constitutive high-light (HL) stress response, a phenotype that we then also confirm in mutants of Arabidopsis thaliana LLM-domain B-GATAs, suggesting that the B-GATAs have a protective role towards HL stress.Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659Peer Reviewe

How do cryptochromes and UVR8 interact in natural and simulated sunlight?

Cryptochromes (CRYs) and UV RESISTANCE LOCUS 8 (UVR8) photoreceptors perceive UV-A/blue (315-500 nm) and UV-B (280-315 nm) radiation in plants, respectively. While the roles of CRYs and UVR8 have been studied in separate controlled-environment experiments, little is known about the interaction between these photoreceptors. Here, Arabidopsis wild-type Ler, CRYs and UVR8 photoreceptor mutants (uvr8-2, cry1cry2 and cry1cry2uvr8-2), and a flavonoid biosynthesis-defective mutant (tt4) were grown in a sun simulator. Plants were exposed to filtered radiation for 17 d or for 6 h, to study the effects of blue, UV-A, and UV-B radiation. Both CRYs and UVR8 independently enabled growth and survival of plants under solar levels of UV, while their joint absence was lethal under UV-B. CRYs mediated gene expression under blue light. UVR8 mediated gene expression under UV-B radiation, and in the absence of CRYs, also under UV-A. This negative regulation of UVR8-mediated gene expression by CRYs was also observed for UV-B. The accumulation of flavonoids was also consistent with this interaction between CRYs and UVR8. In conclusion, we provide evidence for an antagonistic interaction between CRYs and UVR8 and a role of UVR8 in UV-A perception.Peer reviewe

Integrative multi‐omics analyses of date palm (Phoenix dactylifera) roots and leaves reveal how the halophyte land plant copes with sea water

Date palm (Phoenix dactylifera L.) is able to grow and complete its life cycle while being rooted in highly saline soils. Which of the many well-known salt-tolerance strategies are combined to fine-tune this remarkable resilience is unknown. The precise location, whether in the shoot or the root, where these strategies are employed remains uncertain, leaving us unaware of how the various known salt-tolerance mechanisms are integrated to fine-tune this remarkable resilience. To address this shortcoming, we exposed date palm to a salt stress dose equivalent to seawater for up to 4 weeks and applied integrative multi-omics analyses followed by targeted metabolomics, hormone, and ion analyses. Integration of proteomic into transcriptomic data allowed a view beyond simple correlation, revealing a remarkably high degree of convergence between gene expression and protein abundance. This sheds a clear light on the acclimatization mechanisms employed, which depend on reprogramming of protein biosynthesis. For growth in highly saline habitats, date palm effectively combines various salt-tolerance mechanisms found in both halophytes and glycophytes: “avoidance” by efficient sodium and chloride exclusion at the roots, and “acclimation” by osmotic adjustment, reactive oxygen species scavenging in leaves, and remodeling of the ribosome-associated proteome in salt-exposed root cells. Combined efficiently as in P. dactylifera L., these sets of mechanisms seem to explain the palm's excellent salt stress tolerance

Phenotypic responses to drought stress in wheat on genotype, plant and single seed level

Plant phenotyping reveals relationships between measured plant parameters and environmental conditions, thus enabling the study of plant genotype-environment interactions. In our approach we aim to better understand how drought stress during the vegetative phase affects growth and physiological plant responses and thus feeds back on seed traits at harvest. In a greenhouse experiment, nine wheat cultivars selected from the ‘10+ Wheat Genomes Project’ were scanned twice a week for approximately 5 months using the 3D phenotyping system 'Plant Eye'. After harvest, biometric seed traits, such as mass and volume of individual seeds, were phenotyped using the ‘phenoSeeder’. Drought stress resulted in a mean plant biomass reduction by 31%, but also induced genotype-specific responses. The CDC Landmark, CDC Stanley, Norin and Weebil cultivars were generally most sensitive to drought stress, while cv Arina, Cadenza, Chinese Spring, Jagger and Mace showed higher tolerance. Seed characteristics showed high variability among cultivars and plant individuals. Mean values of seed mass ranged from 43 mg (Chinese Spring) to 70 mg (Weebil). Generally, intra-genotype distributions of seed volume and mass were rather wide, with at least a factor two between the values for the smallest and biggest seeds. The drought treatment reduced seed mass and volume only in the cv Chinese Spring and Weebil. Moreover, we did not find any effect of drought stress on seed density. Thus, the drought stress effect on seed traits was different from the response seen in plant biomass and yield. Overall, the applied phenotyping tools allowed for a non-invasive quantification of plant and seed responses on genotype, individual plant and single seed level. We believe that the combination of the different phenotyping approaches, seed classification and seed selection will help to more efficiently identify the genetic basis of complex traits such as drought resistance

Ultraviolet-B component of sunlight stimulates photosynthesis and flavonoid accumulation in variegated Plectranthus coleoides leaves depending on background light

We used variegated Plectranthus coleoides as a model plant with the aim of clarifying whether the effects of realistic ultraviolet-B (UV-B) doses on phenolic metabolism in leaves are mediated by photosynthesis. Plants were exposed to UV-B radiation (0.90Wm(-2)) combined with two photosynthetically active radiation (PAR) intensities [395 and 1350molm(-2)s(-1), low light (LL) and high light (HL)] for 9d in sun simulators. Our study indicates that UV-B component of sunlight stimulates CO2 assimilation and stomatal conductance, depending on background light. UV-B-specific induction of apigenin and cyanidin glycosides was observed in both green and white tissues. However, all the other phenolic subclasses were up to four times more abundant in green leaf tissue. Caffeic and rosmarinic acids, catechin and epicatechin, which are endogenous peroxidase substrates, were depleted at HL in green tissue. This was correlated with increased peroxidase and ascorbate peroxidase activities and increased ascorbate content. The UV-B supplement to HL attenuated antioxidative metabolism and partly recovered the phenolic pool indicating stimulation of the phenylpropanoid pathway. In summary, we propose that ortho-dihydroxy phenolics are involved in antioxidative defence in chlorophyllous tissue upon light excess, while apigenin and cyanidin in white tissue have preferentially UV-screening function.This is the peer-reviewed version of the article: [https://imagine.imgge.bg.ac.rs/handle/123456789/1610

Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity

We studied the specific effects of high photosynthetically active radiation (PAR, 400-700 nm) and ecologically relevant UV-B radiation (0.90 W m(-2)) on antioxidative and phenolic metabolism by exploiting the green-white leaf variegation of Pelargonium zonale plants. This is a suitable model system for examining "source-sink" interactions within the same leaf. High PAR intensity (1350 mu mol m(-2) s(-1)) and UV-B radiation induced different responses in green and white leaf sectors. High PAR intensity had a greater influence on green tissue, triggering the accumulation of phenylpropanoids and flavonoids with strong antioxidative function. Induced phenolics, together with ascorbate, ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) provided efficient defense against potential oxidative pressure. UV-B-induced up-regulation of non-phenolic H2O2 scavengers in green leaf sectors was greater than high PAR-induced changes, indicating a UV-B role in antioxidative defense under light excess; on the contrary, minimal effects were observed in white tissue. However, UV-B radiation had greater influence on phenolics in white leaf sections compared to green ones, inducing accumulation of phenolic glycosides whose function was UV-B screening rather than antioxidative. By stimulation of starch and sucrose breakdown and carbon allocation in the form of soluble sugars from "source" (green) tissue to "sink" (white) tissue, UV-B radiation compensated the absence of photosynthetic activity and phenylpropanoid and flavonoid biosynthesis in white sectors

Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity

We studied the specific effects of high photosynthetically active radiation (PAR, 400-700 nm) and ecologically relevant UV-B radiation (0.90 W m(-2)) on antioxidative and phenolic metabolism by exploiting the green-white leaf variegation of Pelargonium zonale plants. This is a suitable model system for examining "source-sink" interactions within the same leaf. High PAR intensity (1350 mu mol m(-2) s(-1)) and UV-B radiation induced different responses in green and white leaf sectors. High PAR intensity had a greater influence on green tissue, triggering the accumulation of phenylpropanoids and flavonoids with strong antioxidative function. Induced phenolics, together with ascorbate, ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) provided efficient defense against potential oxidative pressure. UV-B-induced up-regulation of non-phenolic H2O2 scavengers in green leaf sectors was greater than high PAR-induced changes, indicating a UV-B role in antioxidative defense under light excess; on the contrary, minimal effects were observed in white tissue. However, UV-B radiation had greater influence on phenolics in white leaf sections compared to green ones, inducing accumulation of phenolic glycosides whose function was UV-B screening rather than antioxidative. By stimulation of starch and sucrose breakdown and carbon allocation in the form of soluble sugars from "source" (green) tissue to "sink" (white) tissue, UV-B radiation compensated the absence of photosynthetic activity and phenylpropanoid and flavonoid biosynthesis in white sectors